CHRNA7 antibody (Extracellular)

Catalog No. ABIN7581857

The Guinea Pig Polyclonal anti-CHRNA7 antibody is suitable to detect CHRNA7 in samples from Rat. It has been validated for WB and IHC.

Quick Overview for CHRNA7 antibody (Extracellular) (ABIN7581857)

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This CHRNA7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CHRNA7 Antibody

Binding Specificity: AA 31-42, Extracellular

Purpose: A Guinea Pig Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)

Sequence: (C)KELVKNYNPL ER

Specificity: Extracellular, N-terminus

Predicted Reactivity: Mouse,human - identical

Characteristics: Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (ABIN7581857) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CHRNA7 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (ABIN7043067, ABIN7044631 and ABIN7044632) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KELVKNYNPLER, corresponding to amino acid residues 31-42 of rat nAChRalpha7

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: (ABIN7235134)

Blocking Peptide: (ABIN7235134)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CHRNA7

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Alternative Name: CHRNA7

Background: NAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7,Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs), muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1. These channel receptors are usually non-selective cation channels activated upon ligand binding which ultimately leads to the depolarization of postsynaptic cell membranes.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore1. However, the α7 subunit mainly forms homomeric functional structures, although functional channels have been observed with its association with α5, β2 or β3 subunits1.Interestingly, the α7 nAChR is the only subunit to be activated by two endogenous ligands: acetylcholine and choline1.All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5. The diversity of these receptors and their functional organization gives rise to unique properties and functions. The α4β2 receptor composition makes up a high affinity nicotinic receptor. In fact, its upregulation (mainly expressed by the increase of functional receptors at the membrane and not expression per se) is responsible for the increased appearance of binding sites following nicotine administration1,3.α7 nAChR seems to be involved in the impairment of sensory gating in schizophrenic individuals. Indeed, many polyphormisms have been detected in the gene promoter of the receptor6. There is also an association of the receptor with nociception as it, along with α2 and α10 subunits are expressed in DRGs, the nociceptive center7.

Gene ID: 25302

UniProt: Q05941

Pathways: Synaptic Membrane