DREAM antibody (Intracellular)

Catalog No. ABIN7581907

This Rabbit Polyclonal antibody specifically detects DREAM in WB. It exhibits reactivity toward Rat.

Quick Overview for DREAM antibody (Intracellular) (ABIN7581907)

Target: DREAM (KCNIP3) (Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DREAM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DREAM Antibody

Binding Specificity: AA 18-31, Intracellular

Purpose: A Rabbit Polyclonal Antibody to KChIP3 (CSEN, DREAM) Subunit

Sequence: (C)SKREGIKWQR PR

Specificity: Cytoplasmic, N-terminus

Predicted Reactivity: Human,mouse - 11,12 amino acid residues identical

Characteristics: Highly specific antibody directed against an epitope of rat Calsenilin. Anti-KChIP3 (CSEN, DREAM) Antibody (ABIN7581907) can be used for western blot analysis. It has been designed to recognize Calsenilin from human mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SKREGIKWQRPR, corresponding to amino acid residues 18-31 of rat KChIP3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for DREAM

Target: DREAM (KCNIP3) (Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))

Alternative Name: KCNIP3

Background: Calsenilin, KCNIP3, KV channel-interacting protein 3, A-type potassium channel modulatory protein 3, DRE-antagonist modulator,Voltage-gated K+ (KV) channels form functional entities by the assembly of four α subunits and auxiliary subunits. Various auxiliary subunits are known to interact with KV channels thereby modulating various properties such as gating, activation and inactivation of the channels as well as influencing trafficking of the channels to the cell's plasma membrane1.KChIPs (KV channel interacting proteins) are cytoplasmic proteins which belong to the neural Ca2+ sensor (NCS) family of Ca2+ binding EF-hand proteins. To date, KChIP1-4 have been identified. All four KChIPs have a conserved C-terminal domain, which has four EF-hand-like Ca2+ binding motifs. The N-terminal region differs among the various KChIPs and attributes different properties regarding the regulation of KV channels1. KChIPs regulate different properties of KV channels such as their cell surface expression (mediated by proper trafficking of the various subunits), channel assembly and gating1-4. Specifically, KChIP2, 3 and 4 strongly regulate the activity of the KV4 channel family in cortical pyramidal neurons5. Furthermore, KChIP knockout mice display an increase in anxiety-like behavior compared to their wild type counterparts6.KChIP1, 3, and 4 are mostly expressed in the brain while KChIP2 is expressed in the heart as well as in the brain4-6.

Gene ID: 65199

UniProt: Q9JM47