MRGPRD antibody (Intracellular)

Catalog No. ABIN7581915

This anti-MRGPRD antibody is a Rabbit Polyclonal antibody detecting MRGPRD in WB, IHC and IF. Suitable for Rat.

Quick Overview for MRGPRD antibody (Intracellular) (ABIN7581915)

Target: MRGPRD (MAS-Related GPR, Member D (MRGPRD))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRGPRD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-MRGPRD Antibody

Binding Specificity: AA 285-299, Intracellular

Purpose: A Rabbit Polyclonal Antibody to MRGPRD (GPCR TGR7) Receptor

Sequence: (C)KSHRLQESLG AVLGR

Specificity: Intracellular, C-terminus

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - identical It will not recognize MRGPRD from human samples

Characteristics: Anti-MRGPRD (GPCR TGR7) Antibody (ABIN7581915) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRD from rat and mouse samples. The antibody will not recognize MRGPRD from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MRGPRD

Target: MRGPRD (MAS-Related GPR, Member D (MRGPRD))

Alternative Name: MRGPRD

Background: Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor,Mas-related G-protein coupled receptor member D (MRGPRD) is expressed in the dorsal root ganglia, specifically in non-peptidergic, small-diameter, IB4+ C-fiber neurons that innervate the outer layers of the mammalian skin. They transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa of the spinal cord.MRGPRD receptor expressing neurons are essential for the full expression of mechanical nociception. Thus, MRGPRD represents an attractive therapeutic target for the development of agents for treating pain1,2.The Mrg family in mice is comprised of six single-copy genes: MrgD, MrgE, MrgF/RTA, MrgG, MrgH/GPR90, and MAS1. In addition, there are three large subfamilies: MrgA, MrgB,and MrgC3.MRGPRD receptor is activated by β-alanine and is able to inhibit KCNQ/M-type potassium channels and to increase excitability of sensory neurons4,5. MRGPRD has the ability to form heterodimers and is encoded by the TGR7 gene. This receptor is co-expressed with two nociceptors, including purinergic receptor (P2X3) and a vanilloid receptor (VR1)5.

Gene ID: 293648

UniProt: Q7TN41