Neurotrophin 3 antibody (Pro-Domain)

Catalog No. ABIN7886734

The Rabbit Polyclonal anti-Neurotrophin 3 antibody (ABIN7886734) specifically detects Neurotrophin 3 in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for Neurotrophin 3 antibody (Pro-Domain) (ABIN7886734)

Target: Neurotrophin 3 (NTF3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurotrophin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Neurotrophin 3 Antibody

Binding Specificity: AA 89-103, Pro-Domain

Purpose: A Rabbit Polyclonal Antibody to proNT-3

Sequence: (C)DTELLRQQRR YNSPR

Cross-Reactivity (Details): The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3.

Predicted Reactivity: Rat,mouse - identical

Characteristics: Highly specific antibody directed against an epitope of human pro. Anti-pro Antibody (ABIN7581927) can be used in western blot and immunohistochemistry applications. It has been designed to recognize pro from rat, mouse and human samples. The antibody does not cross reacts with mature , pro- and mature NGF or pro- and mature BDNF.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor)

Isotype: IgG

Application Details

Application Notes:

WB: 1:300-1:600

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: NTF3

Background:

Synonyms: pro-Neurotrophin 3, proNTF3

Description: Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins. These are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.Until recently, the functional role of the neurotrophin prodomain was thought to assist in the correct folding of the mature protein and the sorting of the neurotrophin into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions. Several studies implicate both proNGF and proBDNF in several physiological and pathological conditions.It remains to be established whether proNT-3 has different biological functions than the mature NT-3 neurotrophin.

Gene ID: 4908

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway