CALCRL antibody (Extracellular) (PE)

Catalog No. ABIN7582012

This Rabbit Polyclonal antibody specifically detects CALCRL in FACS and LCI. It exhibits reactivity toward Human.

Quick Overview for CALCRL antibody (Extracellular) (PE) (ABIN7582012)

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALCRL antibody is conjugated to PE

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CALCRL Antibody (PE)

Binding Specificity: AA 127-140, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Calcitonin Receptor-Like Receptor conjugated to the fluorescent dye R-Phycoerythrin

Sequence: CNVNTHEKVK TALN

Specificity: Extracellular, N-terminus.

Predicted Reactivity: Mouse,rat - 12 out of 14 amino acid residues identical

Characteristics: Anti-CRLR/CALCRL (extracellular) Antibody (ABIN7042979, ABIN7044198 and ABIN7044199) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CALCRL from human, mouse and rat samples. Anti-CRLR/CALCRL (extracellular)-PE Antibody (ABIN7042979, ABIN7044198 and ABIN7044199-PE) is directly conjugated to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: CNVNTHEKVKTALN, corresponding to amino acid residues 127 - 140 of human Calcitonin Receptor-Like Receptor 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582046)

Blocking Peptide: (ABIN7234904)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CALCRL

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Alternative Name: CALCRL

Background: Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR,Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism1.When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor1.CGRP receptor is a heterodimer composed of two proteins: a 7-transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR2.CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines3 and Alzheimer's disease1.

Gene ID: 10203

UniProt: Q16602

Pathways: cAMP Metabolic Process