GABRE antibody (Extracellular) (Atto 633)

Catalog No. ABIN7582029

The Atto 633-conjugated Rabbit Polyclonal anti-GABRE antibody (ABIN7582029) specifically detects GABRE in IHC, IC, IF and LCI. The antibody is reactive with Rat samples.

Quick Overview for GABRE antibody (Extracellular) (Atto 633) (ABIN7582029)

Target: GABRE (gamma-aminobutyric Acid (GABA) A Receptor, epsilon (GABRE))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRE antibody is conjugated to Atto 633

Application: Immunohistochemistry (IHC), Immunochromatography (IC), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-GABRE Antibody (Atto 633)

Binding Specificity: AA 131-144, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GABA(A) ε Receptor Conjugated to the Fluorescent Dye ATTO-633

Sequence: (C)RLRYNDTFET LILH

Specificity: Extracellular, N-terminus

Predicted Reactivity: Mouse - identical, human - 10,14 amino acid residues identical

Characteristics: Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (ABIN7581874) is a highly specific antibody directed against an extracellular epitope of the rat GABA(A) receptor ε subunit. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GABA(A) ε receptor from human, rat and mouse samples. Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody (ABIN7581874)-FR) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody has been tested in live cell imaging and immunohistochemistry applications. It is specially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RLRYNDTFETLILH, corresponding to amino acid residues 131-144 of rat GABRE

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GABRE

Target: GABRE (gamma-aminobutyric Acid (GABA) A Receptor, epsilon (GABRE))

Alternative Name: GABRE

Background: GABA(A) receptor subunit epsilon, Gamma-aminobutyric acid receptor subunit epsilon,GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to the GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ?1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.

Gene ID: 65191

UniProt: Q9ES14

Pathways: Synaptic Membrane