GLP1R antibody (Extracellular) (PE)

Catalog No. ABIN7582031

The Rabbit Polyclonal anti-GLP1R antibody has been validated for FACS and LCI. It is suitable to detect GLP1R in samples from Rat.

Quick Overview for GLP1R antibody (Extracellular) (PE) (ABIN7582031)

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLP1R antibody is conjugated to PE

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GLP1R Antibody (PE)

Binding Specificity: AA 288-301, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GLP1R conjugated to R-Phycoerythrin (R-PE) fluorophore

Sequence: (C)KYLYEDEG(S)WTRNS

Specificity: 2nd extracellular loop.

Predicted Reactivity: Rat,mouse,human - 13,14 amino acid residues identical.

Characteristics: Anti-GLP1R (extracellular) Antibody (ABIN7043219, ABIN7044406 and ABIN7044407) is a highly specific antibody directed against an epitope of the rat glucagon-like peptide 1 receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GLP1R from human, rat, and mouse samples. Anti-GLP1R (extracellular)-PE Antibody (ABIN7043219, ABIN7044406 and ABIN7044407-PE) is directly conjugated to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Negative Control: (ABIN7582046)

Blocking Peptide: (ABIN7235607)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GLP1R

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Alternative Name: GLP1R

Background: Glucagon-like peptide 1 receptor, GLP-1 receptor,Glucagon-like peptide 1 (GLP-1) is a hormone secreted by the intestine following a meal1. This hormone is known to enhance the secretion of insulin, important glucose blood level regulation2,3. In pancreatic β-cells, other than stimulating insulin secretion, GLP-1 action is also important for β-cell mass expansion2. GLP-1 exerts its biological functions through Glucagon-like peptide 1 receptor (GLP-1 receptor), a member of the G-protein coupled receptor superfamily. Like all members, this receptor spans the membrane seven times. GLP-1 receptor couples with Gs, thereby stimulating adenylate cyclase, consequently leading to an increase in cAMP2. The receptor is expressed in various tissues, namely the pancreas, adipose tissue, muscle, heart, gastrointestinal tract and liver. GLP-1 receptor is also found in the central nervous system where it provides a neuroprotective effect4. The glucagon-like peptide 1 system may serve as a therapeutic treatment in diabetes mellitus, in order to maintain β-cell mass expansion2. In addition, the receptor may serve as a therapy target in various neurodegenerative diseases like Alzheimer's and Parkinson's disease4.

Gene ID: 25051

UniProt: P32301

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, cAMP Metabolic Process, Feeding Behaviour