Prostacyclin Receptor antibody (Extracellular) (FITC)

Catalog No. ABIN7582070

The Rabbit Polyclonal anti-Prostacyclin Receptor antibody has been validated for FACS and LCI. It is suitable to detect Prostacyclin Receptor in samples from Rat.

Quick Overview for Prostacyclin Receptor antibody (Extracellular) (FITC) (ABIN7582070)

Target: Prostacyclin Receptor (PTGIR)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prostacyclin Receptor antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Prostacyclin Receptor Antibody (FITC)

Binding Specificity: AA 290-302, Extracellular

Purpose: A Rabbit Polyclonal Antibody to PTGIR (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)TQAIAPDSRE MGD

Specificity: 3rd extracellular loop

Predicted Reactivity: Mouse - identical, human - 11,13 amino acid residues identical

Characteristics: Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (ABIN7043563, ABIN7045122 and ABIN7045123) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize PGI2 from mouse, rat and human samples. Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody (ABIN7043563, ABIN7045122 and ABIN7045123-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize PGI2 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)TQAIAPDSREMGD, corresponding to amino acid residues 290-302 of rat PTGIR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236620)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Prostacyclin Receptor

Target: Prostacyclin Receptor (PTGIR)

Alternative Name: PTGIR

Background: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor, PRIPR,Prostaglandins comprise a family of locally acting hormones, whose synthesis is initiated by the enzyme COX. This pathway is involved in a broad variety of pathological conditions such as cancer, inflammation and hypertension.Prostaglandin I2 receptor (PGI2 receptor) is G-coupled protein receptor (GPCR) with seven hydrophobic transmembrane segments and an extracellular NH2 terminus. Prostaglandin receptors belong to family A, group V of GPCRs. There is significant ligand-binding cross-reactivity between different prostaglandins and different receptors in the family. Within the PG family, receptors share roughly 20-30 % of their sequence and there are 34 identical residues across the family. Twenty of these residues are unique to PG receptors and are not shared by other GPCRs. The unique residues reside mostly in the transmembrane region and in the second extracellular loop region. Both of these regions are implicated in ligand binding. The PGI2 receptor signals primarily via stimulation of cAMP generation but through PIP2 as well despite a much higher agonist concentration required for PIP2 hydrolysis.PGI2 receptor mRNA is highly expressed in the human heart, aorta, kidney, liver and lung tissues and in mice thymus, heart and spleen1. Mice lacking PGI2 receptor have been shown to have a reduced inflammation response and a significant decrease in clinical arthritis. In addition, human patients with a dysfunctional PGI2 receptor variant exhibit an enhanced atherothrombotic phenotype with higher incidence of triple vessel coronary disease and cardiovascular events2.

Gene ID: 292661

UniProt: P43253

Pathways: cAMP Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling