MAFK antibody (AA 1-156)

Catalog No. ABIN7596043

Product Details anti-MAFK Antibody

Target: MAFK (Transcription Factor MafK (MAFK))

Binding Specificity: AA 1-156

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAFK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Human MafK antibody

Purification: protein-A affinity chromatography

Immunogen: Recombinant human MAFK (1-156aa) purified from E. coli

Clone: AT2F7

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycero

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details for MAFK

Target: MAFK (Transcription Factor MafK (MAFK))

Alternative Name: MafK (MAFK Products)

Background: The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locuscontrol regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by thetranscription factor NFE2 (nuclear factor erythroid-2). NFE2 DNA-binding activity consists of aheterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack aputative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize amongthemselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger)basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerizewith Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteinsis primarily expressed during development in mesenchymal and hematopoietic cells and neurons.

NCBI Accession: NP_002351