Nanog antibody (AA 1-191)

Catalog No. ABIN7599059

The Rabbit Polyclonal anti-Nanog antibody is suitable to detect Nanog in samples from Mouse and Rat. It has been validated for WB, ELISA, IF, FACS and ICC.

Quick Overview for Nanog antibody (AA 1-191) (ABIN7599059)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Nanog Antibody

Binding Specificity: AA 1-191

Purpose: Anti-Nanog Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Nanog Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Nanog recombinant protein (Position: M1-A191).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog

Background:

Background: NANOG (pron. nanOg) is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. In humans, this protein is encoded by the NANOG gene. It is mapped to 12p13.31. NANOG is thought to be a key factor in maintaining pluripotency. Moreover, NANOG is also thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. The < a href=https://www.bosterbio.com/human-nanog-recombinant-protein-protq9h9s0-boster.html style=color:#EA8D28>NANOG protein has been found to be a transcriptional activator for the Rex1 promoter, playing a key role in sustaining Rex1 expression. Knockdown of NANOG in embryonic stem cells results in a reduction of Rex1 expression, while forced expression of NANOG stimulates Rex1 expression.

Gene Full Name: Nanog homeobox

Molecular Weight: 37 kDa

Gene ID: 71950

UniProt: Q80Z64

Pathways: Stem Cell Maintenance