NUF2 antibody (AA 1-297)

Catalog No. ABIN7599236

The Rabbit Polyclonal anti-NUF2 antibody has been validated for WB and ELISA. It is suitable to detect NUF2 in samples from Human.

Quick Overview for NUF2 antibody (AA 1-297) (ABIN7599236)

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NUF2 Antibody

Binding Specificity: AA 1-297

Purpose: Anti-NUF2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NUF2 Antibody Picoband® (ABIN7599236). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NUF2 recombinant protein (Position: M1-Q297).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. DeLuca, J. G., Moree, B., Hickey, J. M., Kilmartin, J. V., Salmon, E. D. hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J. Cell Biol. 159: 549-555, 2002. 2. Liu, D., Ding, X., Du, J., Cai, X., Huang, Y., Ward, T., Shaw, A., Yang, Y., Hu, R., Jin, C., Yao, X. Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J. Biol. Chem. 282: 21415-21424, 2007. 3. Nabetani, A., Koujin, T., Tsutsumi, C., Haraguchi, T., Hiraoka, Y. A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint. Chromosoma 110: 322-334, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NUF2

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Alternative Name: NUF2

Background:

Synonyms: S-arrestin, 48 kDa protein, Retinal S-antigen, S-AG, Rod photoreceptor arrestin, SAG,

Tissue Specificity: Retina and pineal gland.

Background: Kinetochore protein Nuf2 is a protein that in humans is encoded by the NUF2 gene. This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.

Molecular Weight: 54 kDa

Gene ID: 83540

Pathways: Maintenance of Protein Location