SKIL antibody (AA 1-504)

Catalog No. ABIN7599455

The Rabbit Polyclonal anti-SKIL antibody is suitable to detect SKIL in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF, FACS and ICC.

Quick Overview for SKIL antibody (AA 1-504) (ABIN7599455)

Target: SKIL (SKI-Like Oncogene (SKIL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKIL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SKIL Antibody

Binding Specificity: AA 1-504

Purpose: Anti-SnoN/SNO/SKIL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SnoN/SNO/SKIL Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SnoN/SNO/SKIL recombinant protein (Position: M1-V504).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SKIL

Target: SKIL (SKI-Like Oncogene (SKIL))

Alternative Name: SKIL

Background:

Background: Ski-like protein is a protein that in humans is encoded by the SKIL gene. The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene.

Gene Full Name: SKI like proto-oncogene

Molecular Weight: 77 kDa

Gene ID: 6498

UniProt: P12757

Pathways: Positive Regulation of Response to DNA Damage Stimulus