GLS2 antibody (AA 1-602)

Catalog No. ABIN7599493

This Rabbit Polyclonal antibody specifically detects GLS2 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for GLS2 antibody (AA 1-602) (ABIN7599493)

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GLS2 Antibody

Binding Specificity: AA 1-602

Purpose: Anti-GLS2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GLS2 Antibody Picoband® (ABIN7599493). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GLS2 recombinant protein (Position: M1-V602).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gomez-Fabre, P. M., Aledo, J. C., del Castillo-Olivares, A., Alonso, F. J., Nunez de Castro, I., Campos, J. A., Marquez, J. Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase. Biochem. J. 345: 365-375, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GLS2

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Alternative Name: GLS2

Background:

Synonyms: Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG, CKAP5, KIAA0097

Tissue Specificity: Expressed in fetal brain. Highly expressed in brain and placenta. Lower levels in heart, liver, thymus, kidney and lung. Located to endothelial cells and neuronal cells of the suprachiasmatic nucleus (SCN). Also detected in endothelial cells of the heart, lung and kidney. In the brain, specifically expressed in the thalamus, hippocampus and amygdala.

Background: Glutaminase 2 (liver, mitochondrial) is a protein that in humans is encoded by the GLS2 gene. The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms.

Molecular Weight: 66 kDa

Gene ID: 27165

Pathways: Dicarboxylic Acid Transport, Warburg Effect