TMPRSS6 antibody (AA 1-759)

Catalog No. ABIN7599541

This Rabbit Polyclonal antibody specifically detects TMPRSS6 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TMPRSS6 antibody (AA 1-759) (ABIN7599541)

Target: TMPRSS6 (Transmembrane Protease, serine 6 (TMPRSS6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMPRSS6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TMPRSS6 Antibody

Binding Specificity: AA 1-759

Purpose: Anti-Matriptase 2/TMPRSS6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Matriptase 2/TMPRSS6 Antibody Picoband® (ABIN7599541). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Matriptase 2/TMPRSS6 recombinant protein (Position: M1-Q759).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Du, X., She, E., Gelbart, T., Truksa, J., Lee, P., Xia, Y., Khovananth, K., Mudd, S., Mann, N., Moresco, E. M. Y., Beutler, E., Beutler, B. The serine protease TMPRSS6 is required to sense iron deficiency. Science 320: 1088-1092, 2008. 2. Finberg, K. E., Heeney, M. M., Campagna, D. R., Aydinok, Y., Pearson, H. A., Hartman, K. R., Mayo, M. M., Samuel, S. M., Strouse, J. J., Markianos, K., Andrews, N. C., Fleming, M. D. Mutations in TMPRSS6 cause iron-refractory iron deficiency anemia (IRIDA). Nature Genet. 40: 569-571, 2008. 3. Guillem, F., Kannengiesser, C., Oudin, C., Lenoir, A., Matak, P., Donadieu, J., Isidor, B., Mechinaud, F., Aguilar-Martinez, P., Beaumont, C., Vaulont, S., Grandchamp, B., Nicolas, G. Inactive matriptase-2 mutants found in IRIDA patients still repress hepcidin in a transfection assay despite having lost their serine protease activity. Hum. Mutat. 33: 1388-1396, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TMPRSS6

Target: TMPRSS6 (Transmembrane Protease, serine 6 (TMPRSS6))

Alternative Name: TMPRSS6

Background:

Synonyms: Protein sprouty homolog 2, Spry-2, SPRY2

Tissue Specificity: Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.

Background: Transmembrane protease, serine 6 (also known as matriptase-2) is an enzyme that in humans is encoded by the TMPRSS6 gene. The protein encoded by this gene is a type II transmembrane serine proteinase that is found attached to the cell surface. The encoded protein may be involved in matrix remodeling processes in the liver. Alternative splicing results in multiple transcript variants.

Molecular Weight: 90 kDa, 95 kDa

Gene ID: 164656

Pathways: Transition Metal Ion Homeostasis