RHOB antibody (AA 117-158)

Catalog No. ABIN7599782

This Rabbit Polyclonal antibody specifically detects RHOB in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RHOB antibody (AA 117-158) (ABIN7599782)

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RHOB Antibody

Binding Specificity: AA 117-158

Purpose: Anti-RHOB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RHOB Antibody Picoband® (ABIN7599782). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RHOB recombinant protein (Position: N117-E158).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Cannizzaro, L. A., Madaule, P., Hecht, F., Axel, R., Croce, C. M., Huebner, K. Chromosome localization of human ARH genes, a ras-related gene family. Genomics 6: 197-203, 1990. 2. Chardin, P., Madaule, P., Tavitian, A. Coding sequence of human rho cDNAs clone 6 and clone 9. Nucleic Acid Res. 16: 2717 only, 1988. 3. Madaule, P., Axel, R. A novel ras-related gene family. Cell 41: 31-40, 1985.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RHOB

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Alternative Name: RHOB

Background:

Synonyms: Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6, RHOB, ARH6, ARHB

Tissue Specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

Background: Ras homolog gene family, member B, also known as RHOB, is a protein which in humans is encoded by the RHOB gene. This gene is mapped to 2p24.1. It is a member of the Rho GTP-binding protein family. And RHOB has been shown to interact with CIT, ARHGEF3, ARHGDIG and RHPN2. RHOB plays a negative role in tumorigenesis as deletion causes tumor formation. Also, it serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis.

Molecular Weight: 22 kDa

Gene ID: 388

UniProt: P62745