LARP1 antibody (AA 123-401)

Catalog No. ABIN7599861

Product Details anti-LARP1 Antibody

Target: LARP1 (La Ribonucleoprotein Domain Family, Member 1 (LARP1))

Binding Specificity: AA 123-401

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LARP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Anti-LARP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-LARP1 Antibody Picoband® (ABIN7599861). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LARP1 recombinant protein (Position: K123-E401).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chauvet, S., Maurel-Zaffran, C., Miassod, R., Jullien, N., Pradel, J., Aragnol, D. dlarp, a new candidate Hox target in Drosophila whose orthologue in mouse is expressed at sites of epithelium/mesenchymal interactions. Dev. Dyn. 218: 401-413, 2000. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/16/2008. 3. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 277-286, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LARP1

Target: LARP1 (La Ribonucleoprotein Domain Family, Member 1 (LARP1))

Alternative Name: LARP1 (LARP1 Products)

Background:

Synonyms: Interferon regulatory factor 9,IRF-9,IFN-alpha-responsive transcription factor subunit,ISGF3 p48 subunit,Interferon-stimulated gene factor 3 gamma,ISGF-3 gamma,Transcriptional regulator ISGF3 subunit gamma,IRF9,ISGF3G,

Tissue Specificity: Leukocytes.

Background: La-related protein 1 (LARP1) is a 150 kDa protein that in humans is encoded by the LARP1 gene. La-related protein 1 (LARP1) is a ubiquitously expressed RNA binding protein that promotes both global and specific mRNA translation in cells. LARP1 belongs to the La-related protein family and contains two RNA binding domains, a La motif (LAM), and a neighboring RNA recognition motif-like (RRM-L) domain. Research studies indicate that LARP1 acts downstream of mTORC1 to facilitate cell proliferation and growth by promoting global mRNA translation and translation of mRNAs containing a 5'Terminal Oligo-Pyrimidine (5'TOP) motif, which code for translational machinery components. At the molecular level, LARP1 associates with 5'TOP mRNAs and multiple translation machinery components to positively regulate translation. Additional studies show that LARP1 expression is upregulated in hepatocellular carcinoma (HCC) patients and that high LARP1 expression in HCC negatively correlates with survival rate.

Molecular Weight: 150 kDa

Gene ID: 23367

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection