Girdin antibody (AA 1231-1836)

Catalog No. ABIN7599868

This anti-Girdin antibody is a Rabbit Polyclonal antibody detecting Girdin in ELISA, WB and FACS. Suitable for Human and Rat.

Quick Overview for Girdin antibody (AA 1231-1836) (ABIN7599868)

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Girdin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Girdin Antibody

Binding Specificity: AA 1231-1836

Purpose: Anti-CCDC88A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CCDC88A Antibody Picoband® (ABIN7599868). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CCDC88A recombinant protein (Position: K1231-D1836).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Anai, M., Shojima, N., Katagiri, H., Ogihara, T., Sakoda, H., Onishi, Y., Ono, H., Fujishiro, M., Fukushima, Y., Horike, N., Viana, A., Kikuchi, M., Noguchi, N., Takahashi, S., Takata, K., Oka, Y., Uchijima, Y., Kurihara, H., Asano, T. A novel protein kinase B (PKB)/AKT-binding protein enhances PKB kinase activity and regulates DNA synthesis. J. Biol. Chem. 280: 18525-18535, 2005. 2. Enomoto, A., Asai, N., Namba, T., Wang, Y., Kato, T., Tanaka, M., Tatsumi, H., Taya, S., Tsuboi, D., Kuroda, K., Kaneko, N., Sawamoto, K., Miyamoto, R., Jijiwa, M., Murakumo, Y., Sokabe, M., Seki, T., Kaibuchi, K., Takahashi, M. Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus. Neuron 63: 774-787, 2009. 3. Enomoto, A., Murakami, H., Asai, N., Morone, N., Watanabe, T., Kawai, K., Murakumo, Y., Usukura, J., Kaibuchi, K., Takahashi, M. Akt/PKB regulates actin organization and cell motility via Girdin/APE. Dev. Cell. 9: 389-402, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Girdin

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Alternative Name: CCDC88A

Background:

Synonyms: CD48 antigen,B-lymphocyte activation marker BLAST-1,BCM1 surface antigen,Leukocyte antigen MEM-102,SLAM family member 2,SLAMF2,Signaling lymphocytic activation molecule 2,TCT.1,CD48,CD48,BCM1, BLAST1,

Background: Girdin is a protein that in humans is encoded by the CCDC88A gene. This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 240 kDa

Gene ID: 55704

UniProt: Q3V6T2