MECOM antibody (AA 125-743)

Catalog No. ABIN7599882

The Rabbit Polyclonal anti-MECOM antibody has been validated for WB, ELISA, IF, FACS and ICC. It is suitable to detect MECOM in samples from Human.

Quick Overview for MECOM antibody (AA 125-743) (ABIN7599882)

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECOM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-MECOM Antibody

Binding Specificity: AA 125-743

Purpose: Anti-EVI1/MECOM Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EVI1/MECOM Antibody Picoband® (ABIN7599882). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EVI1/MECOM recombinant protein (Position: E125-D743).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Buonamici, S., Li, D., Chi, Y., Zhao, R., Wang, X., Brace, L., Ni, H., Saunthararajah, Y., Nucifora, G. EVI1 induces myelodysplastic syndrome in mice. J. Clin. Invest. 114: 713-719, 2004. Note: Erratum: J. Clin. Invest. 115: 2296 only, 2005. 2. Gross, M. B. Personal Communication. Baltimore, Md. 3/16/2018. 3. Morishita, K., Parganas, E., Bartholomew, C., Sacchi, N., Valentine, M. B., Raimondi, S. C., Le Beau, M. M., Ihle, J. N. The human Evi-1 gene is located on chromosome 3q24-q28 but is not rearranged in three cases of acute nonlymphocytic leukemias containing t(3,5)(q25,q34) translocations. Oncogene Res. 5: 221-231, 1990.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MECOM

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Alternative Name: MECOM

Background:

Synonyms: Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: MDS1 and EVI1 complex locus protein EVI1 (MECOM) also known as ecotropic virus integration site 1 protein homolog (EVI-1) or positive regulatory domain zinc finger protein 3 (PRDM3) is a protein that in humans is encoded by the MECOM gene. The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene.

Molecular Weight: 138 kDa

Gene ID: 2122

UniProt: Q03112