FANCA antibody (AA 1272-1439)

Catalog No. ABIN7599897

The Rabbit Polyclonal anti-FANCA antibody has been validated for WB, ELISA and FACS. It is suitable to detect FANCA in samples from Rat and Mouse.

Quick Overview for FANCA antibody (AA 1272-1439) (ABIN7599897)

Target: FANCA (Fanconi Anemia Group A Protein (FANCA))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FANCA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-FANCA Antibody

Binding Specificity: AA 1272-1439

Purpose: Anti-Fanca/FAA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Fanca/FAA Antibody Picoband® (ABIN7599897). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Fanca/FAA recombinant protein (Position: E1272-F1439).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Abu-Issa, R., Eichele, G., Youssoufian, H. Expression of the Fanconi anemia group A gene (Fanca) during mouse embryogenesis. Blood 94: 818-824, 1999. 2. Bouchlaka, C., Abdelhak, S., Amouri, A., Ben Abid, H., Hadiji, S., Frikha, M., Ben Othman, T., Amri, F., Ayadi, H., Hachicha, M., Rebai, A., Saad, A., Dellagi, K., Tunisian Fanconi Anemia Study Group. Fanconi anemia in Tunisia: high prevalence of group A and identification of new FANCA mutations. J. Hum. Genet. 48: 352-361, 2003. 3. Castella, M., Pujol, R., Callen, E., Trujillo, J. P., Casado, J. A., Gille, H., Lach, F. P., Auerbach, A. D., Schindler, D., Benitez, J., Porto, B., Ferro, T., and 20 others. Origin, functional role, and clinical impact of Fanconi anemia FANCA mutations. Blood 117: 3759-3769, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FANCA

Target: FANCA (Fanconi Anemia Group A Protein (FANCA))

Alternative Name: Fanca

Background:

Synonyms: DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5, CDC46

Tissue Specificity: Ubiquitous.

Background: Fanconi anaemia, complementation group A, also known as FAA, FACA and FANCA, is a protein which in humans is encoded by the FANCA gene. Acts upstream of or within several processes, including gonad development, regulation of CD40 signaling pathway, and regulation of regulatory T cell differentiation. Predicted to be located in nucleoplasm. Predicted to be part of Fanconi anaemia nuclear complex. Is expressed in several structures, including gut, liver, lung, reproductive system, and spleen. Used to study Fanconi anemia complementation group A. Human ortholog(s) of this gene implicated in several diseases, including Fanconi anemia (multiple), acute myeloid leukemia, esophagus squamous cell carcinoma, ovarian disease (multiple), and skin melanoma. Orthologous to human FANCA (FA complementation group A).

Molecular Weight: 180 kDa

Gene ID: 14087

Pathways: DNA Damage Repair