CENPF antibody (AA 129-3078)

Catalog No. ABIN7599912

The Rabbit Polyclonal anti-CENPF antibody is suitable to detect CENPF in samples from Human. It has been validated for ELISA, WB, IF, ICC and FACS.

Quick Overview for CENPF antibody (AA 129-3078) (ABIN7599912)

Target: CENPF (Centromere Protein F (CENPF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPF antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-CENPF Antibody

Binding Specificity: AA 129-3078

Purpose: Anti-CENPF Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CENPF Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CENPF recombinant protein (Position: Q129-R3078).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENPF

Background:

Background: Centromere protein F is a protein that in humans is encoded by the CENPF gene. This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.

Gene Full Name: centromere protein F

Molecular Weight: 358 kDa

Gene ID: 1063

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection