Golgin B1 (GOLGB1) (AA 153-950) antibody

Catalog No. ABIN7600139

This Rabbit Polyclonal antibody specifically detects in WB, ELISA, FACS, IHC, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for Golgin B1 (GOLGB1) (AA 153-950) antibody (ABIN7600139)

Target: Golgin B1 (GOLGB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 153-950

Purpose: Anti-Giantin/GOLGB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Giantin/GOLGB1 Antibody Picoband® (ABIN7600139). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Giantin/GOLGB1 recombinant protein (Position: L153-E950).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.5-1 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 4/17/2014. 2. Lan, Y., Zhang, N., Liu, H., Xu, J., Jiang, R. Golgb1 regulates protein glycosylation and is crucial for mammalian palate development. Development 143: 2344-2355, 2016. 3. Linstedt, A. D., Hauri, H.-P. Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa. Molec. Biol. Cell 4: 679-693, 1993.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Golgin B1 (GOLGB1)

Alternative Name: GOLGB1

Background:

Synonyms: Transforming growth factor beta activator LRRC32, Garpin, Glycoprotein A repetitions predominant, GARP, Leucine-rich repeat-containing protein 32, LRRC32, D11S833E

Tissue Specificity: Heart, placenta, skeletal muscle, kidney, lung and pancreas.

Background: Giantin or Golgin subfamily B member 1 is a protein that in humans is encoded by the GOLGB1 gene. GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family. GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton. It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis. GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome. Human GOLGB1 is 3259 amino acids (aa) in length, and over aa 1596-1702, shares 92 % and 91 % sequence identity with its mouse and rat orthologs, respectively.

Molecular Weight: 376 kDa

Gene ID: 2804

UniProt: Q14789

Pathways: SARS-CoV-2 Protein Interactome