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SWAP70 antibody (AA 161-292)

The Rabbit Polyclonal anti-SWAP70 antibody has been validated for WB, ELISA, IHC, IF and FACS. It is suitable to detect SWAP70 in samples from Human.
Catalog No. ABIN7600201

Quick Overview for SWAP70 antibody (AA 161-292) (ABIN7600201)

Target

See all SWAP70 Antibodies
SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This SWAP70 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • Binding Specificity

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    AA 161-292

    Purpose

    Anti-SWAP70 Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-SWAP70 Antibody Picoband® (ABIN7600201). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human SWAP70 recombinant protein (Position: H161-K292).

    Isotype

    IgG
  • Application Notes

    Western blot, 0.5-1 μg/mL, Human
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
    Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Borggrefe, T., Masat, L., Wabl, M., Riwar, B., Cattoretti, G., Jessberger, R. Cellular, intracellular, and developmental expression patterns of murine SWAP-70. Europ. J. Immun. 29: 1812-1822, 1999. 2. Borggrefe, T., Wabl, M., Akhmedov, A. T., Jessberger, R. A B-cell-specific DNA recombination complex. J. Biol. Chem. 273: 17025-17035, 1998. 3. Masat, L., Caldwell, J., Armstrong, R., Khoshnevisan, H., Jessberger, R., Herndier, B., Wabl, M., Ferrick, D. Association of SWAP-70 with the B cell antigen receptor complex. Proc. Nat. Acad. Sci. 97: 2180-2184, 2000.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))

    Alternative Name

    SWAP70

    Background

    Synonyms: Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, AFB1-AR 1, Aldoketoreductase 7, Succinic semialdehyde reductase, SSA reductase, AKR7A2, AFAR, AFAR1, AKR7

    Tissue Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.

    Background: SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators, in dendritic cells, it regulates the appearance of MHC II, and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96 % aa identity with mouse SWAP70.

    Molecular Weight

    69 kDa

    Gene ID

    23075

    Pathways

    Production of Molecular Mediator of Immune Response
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