SWAP70 antibody (AA 161-292)
Quick Overview for SWAP70 antibody (AA 161-292) (ABIN7600201)
Target
See all SWAP70 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 161-292
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Purpose
- Anti-SWAP70 Antibody Picoband®
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Cross-Reactivity (Details)
- No cross-reactivity with other proteins.
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Characteristics
- Anti-SWAP70 Antibody Picoband® (ABIN7600201). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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Purification
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human SWAP70 recombinant protein (Position: H161-K292).
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Isotype
- IgG
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Application Notes
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Western blot, 0.5-1 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Borggrefe, T., Masat, L., Wabl, M., Riwar, B., Cattoretti, G., Jessberger, R. Cellular, intracellular, and developmental expression patterns of murine SWAP-70. Europ. J. Immun. 29: 1812-1822, 1999. 2. Borggrefe, T., Wabl, M., Akhmedov, A. T., Jessberger, R. A B-cell-specific DNA recombination complex. J. Biol. Chem. 273: 17025-17035, 1998. 3. Masat, L., Caldwell, J., Armstrong, R., Khoshnevisan, H., Jessberger, R., Herndier, B., Wabl, M., Ferrick, D. Association of SWAP-70 with the B cell antigen receptor complex. Proc. Nat. Acad. Sci. 97: 2180-2184, 2000. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Concentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Storage
- 4 °C,-20 °C
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Storage Comment
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))
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Alternative Name
- SWAP70
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Background
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Synonyms: Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, AFB1-AR 1, Aldoketoreductase 7, Succinic semialdehyde reductase, SSA reductase, AKR7A2, AFAR, AFAR1, AKR7
Tissue Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.
Background: SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators, in dendritic cells, it regulates the appearance of MHC II, and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96 % aa identity with mouse SWAP70.
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Molecular Weight
- 69 kDa
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Gene ID
- 23075
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Pathways
- Production of Molecular Mediator of Immune Response
Target
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