ITCH antibody (AA 17-358)

Catalog No. ABIN7600263

This Mouse Monoclonal antibody specifically detects ITCH in WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ITCH antibody (AA 17-358) (ABIN7600263)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 5E12

Product Details anti-ITCH Antibody

Binding Specificity: AA 17-358

Purpose: Anti-ITCH/AIP4 Antibody Picoband® (monoclonal, 5E12)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ITCH/AIP4 Antibody Picoband® (monoclonal, 5E12) (ABIN7600263). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ITCH/AIP4 recombinant protein (Position: K17-E358).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human
1. Dorjbal B, Derse D, Lloyd P, Soheilian F, Nagashima K, Heidecker G. ""The role of ITCH protein in human T-cell leukemia virus type 1 release"". J Biol Chem, 2011 Sep 9. 2. Rossi M, Inoue S, Walewska R, Knight RA, Dyer MJ, Cohen GM, Melino G. ""Caspase cleavage of Itch in chronic lymphocytic leukemia cells"". Biochem Biophys Res Commun, 2009 Feb 13.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH

Background:

Synonyms: Serine/threonine-protein kinase PLK1, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13, PLK1, PLK

Tissue Specificity: Placenta and colon.

Background: ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 103 kDa

Gene ID: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events