SMARCA2 antibody (AA 181-624)

Catalog No. ABIN7600366

The Mouse Monoclonal anti-SMARCA2 antibody is suitable to detect SMARCA2 in samples from Human. It has been validated for WB, ICC and IF.

Quick Overview for SMARCA2 antibody (AA 181-624) (ABIN7600366)

Target: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMARCA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 4E8

Product Details anti-SMARCA2 Antibody

Binding Specificity: AA 181-624

Purpose: Anti-SMARCA2/BRM Antibody (monoclonal, 4E8)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SMARCA2/BRM Antibody (monoclonal, 4E8) (ABIN7600366). Tested in IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SMARCA2/BRM recombinant protein (Position: Q181-N624).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SMARCA2

Target: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Alternative Name: SMARCA2

Background:

Synonyms: Probable global transcription activator SNF2L2, ATP-dependent helicase SMARCA2, BRG1-associated factor 190B, BAF190B, Protein brahma homolog, hBRM, SNF2-alpha, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2, SMARCA2, BAF190B, BRM, SNF2A, SNF2L2

Tissue Specificity: Widely expressed in fetal and adult tissues.

Background: Probable global transcription activator SNF2L2 is a protein that in humans is encoded by the SMARCA2 gene. It is mapped to 9p24.3. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Molecular Weight: 210 kDa

Gene ID: 6595

UniProt: P51531