Prosaposin antibody (AA 182-479)

Catalog No. ABIN7600369

The Rabbit Polyclonal anti-Prosaposin antibody has been validated for WB, IHC, ELISA, IF, ICC and FACS. It is suitable to detect Prosaposin in samples from Human.

Quick Overview for Prosaposin antibody (AA 182-479) (ABIN7600369)

Target: Prosaposin (PSAP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prosaposin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Prosaposin Antibody

Binding Specificity: AA 182-479

Purpose: Anti-PSAP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PSAP Antibody Picoband® (ABIN7600369). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PSAP recombinant protein (Position: Q182-I479).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Azuma, N., O'Brien, J. S., Moser, H. W., Kishimoto, Y. Stimulation of acid ceramidase activity by saposin D. Arch. Biochem. Biophys. 311: 354-357, 1994. 2. Bar-Am, I., Avivi, L., Horowitz, M. Assignment of the human prosaposin gene (PSAP) to 10q22.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 72: 316-318, 1996. 3. Bradova, V., Smid, F., Ulrich-Bott, B., Roggendorf, W., Paton, B. C., Harzer, K. Prosaposin deficiency: further characterization of the sphingolipid activator protein-deficient sibs. Multiple glycolipid elevations (including lactosylceramidosis), partial enzyme deficiencies and ultrastructure of the skin in this generalized sphingolipid storage disease. Hum. Genet. 92: 143-152, 1993.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Prosaposin

Target: Prosaposin (PSAP)

Alternative Name: PSAP

Background:

Synonyms: Tafazzin, Protein G4.5, TAZ, EFE2, G4.5

Tissue Specificity: High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N-terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes.

Background: This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Molecular Weight: 70 kDa

Gene ID: 5660

UniProt: P07602

Pathways: Positive Regulation of Endopeptidase Activity