GLIS3 antibody (AA 184-765)

Catalog No. ABIN7600381

The Rabbit Polyclonal anti-GLIS3 antibody has been validated for WB and ELISA. It is suitable to detect GLIS3 in samples from Human, Mouse and Rat.

Quick Overview for GLIS3 antibody (AA 184-765) (ABIN7600381)

Target: GLIS3 (GLIS Family Zinc Finger 3 (GLIS3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLIS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GLIS3 Antibody

Binding Specificity: AA 184-765

Purpose: Anti-GLIS3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GLIS3 Antibody Picoband® (ABIN7600381). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GLIS3 recombinant protein (Position: A184-P765).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Beak, J. Y., Kang, H. S., Kim, Y.-S., Jetten, A. M. Functional analysis of the zinc finger and activation domains of Glis3 and mutant Glis3(NDH1). Nucleic Acids Res. 36: 1690-1702, 2008. 2. Dimitri, P., Habeb, A. M., Gurbuz, F., Millward, A., Wallis, S., Moussa, K., Akcay, T., Taha, D., Hogue, J., Slavotinek, A., Wales, J. K. H., Shetty, A., Hawkes, D., Hattersley, A. T., Ellard, S., De Franco, E. Expanding the clinical spectrum associated with GLIS3 mutations. J. Clin. Endocr. Metab. 100: E1362-E1369, 2015. Note: Electronic Article. Erratum: J. Clin. Endocr. Metab. 100: 4685 only, 2015. 3. Dimitri, P., Warner, J. T., Minton, J. A. L., Patch, A. M., Ellard, S., Hattersley, A. T., Barr, S., Hawkes, D., Wales, J. K., Gregory, J. W. Novel GLIS3 mutations demonstrate an extended multisystem phenotype. Europ. J. Endocr. 164: 437-443, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GLIS3

Target: GLIS3 (GLIS Family Zinc Finger 3 (GLIS3))

Alternative Name: GLIS3

Background:

Synonyms: Tissue factor pathway inhibitor 2,TFPI-2,Placental protein 5,PP5,TFPI2,

Tissue Specificity: Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.

Background: Zinc finger protein GLI3 is a protein that in humans is encoded by the GLI3 gene. This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined.

Molecular Weight: 84 kDa

Gene ID: 169792