AGRN antibody (AA 1864-2068)

Catalog No. ABIN7600387

The Rabbit Polyclonal anti-AGRN antibody has been validated for IHC, WB, ELISA, ICC, IF and FACS. It is suitable to detect AGRN in samples from Human, Rat and Mouse.

Quick Overview for AGRN antibody (AA 1864-2068) (ABIN7600387)

Target: AGRN (Agrin (AGRN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGRN antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-AGRN Antibody

Binding Specificity: AA 1864-2068

Purpose: Anti-Agrin/AGRN Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Agrin/AGRN Picoband® Antibody (ABIN7600387). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Agrin/AGRN recombinant protein (Position: S1864-P2068).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bassat, E., Mutlak, Y. E., Genzelinakh, A., Shadrin, I. Y., Umansky, K. B., Yifa, O., Kain, D., Rajchman, D., Leach, J., Bassat, D. R., Udi, Y., Sarig, R., Sagi, I., Martin, J. F., Bursac, N., Cohen, S., Tzahor, E. The extracellular matrix protein agrin promotes heart regeneration in mice. Nature 547: 179-184, 2017. 2. Burgess, R. W., Nguyen, Q.T., Son, Y. J., Lichtman, J. W., Sanes, J. R. Alternatively spliced isoforms of nerve- and muscle-derived agrin: their roles at the neuromuscular junction. Neuron 23: 33-44, 1999. 3. Campanelli, J. T., Hoch, W., Rupp, F., Kreiner, T., Scheller, R. H. Agrin mediates cell contact-induced acetylcholine receptor clustering. Cell 67: 909-916, 1991.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AGRN

Target: AGRN (Agrin (AGRN))

Alternative Name: AGRN

Background:

Synonyms: Agrin, Agrin N-terminal 110 kDa subunit, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment, C90, Agrin C-terminal 22 kDa fragment, C22, AGRN, AGRIN

Tissue Specificity: Brain specific. Detected in neuronal cells.

Background: Agrin is a protein that in humans is encoded by the AGRN gene. It is mapped to 1p36.33. This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 205-217 kDa

Gene ID: 375790

UniProt: O00468

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development