C5 antibody (AA 19-294)

Catalog No. ABIN7600410

This Rabbit Polyclonal antibody specifically detects C5 in WB, ELISA and FACS. It exhibits reactivity toward Mouse and Rat.

Quick Overview for C5 antibody (AA 19-294) (ABIN7600410)

Target: C5 (Complement Component 5 (C5))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-C5 Antibody

Binding Specificity: AA 19-294

Purpose: Anti-C5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-C5 Antibody Picoband® (ABIN7600410). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse C5 recombinant protein (Position: Q19-D294).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Arbore, G., West, E. E., Spolski, R., Robertson, A. A. B., Klos, A., Rheinheimer, C., Dutow, P., Woodruff, T. M., Yu, Z. X., O'Neill, L. A., Coll, R. C., Sher, A., and 10 others. T helper 1 immunity requires complement-driven NLRP3 inflammasome activity in CD4+ T cells. Science 352: aad1210, 2016. Note: Electronic Article. 2. Carney, D. F., Haviland, D. L., Noack, D., Wetsel, R. A., Vik, D. P., Tack, B. F. Structural aspects of the human C5 gene: intron/exon organization, 5-prime-flanking region features, and characterization of two truncated cDNA clones. J. Biol. Chem. 266: 18786-18791, 1991. 3. Collaborative Study on the Genetics of Asthma. A genome-wide search for asthma susceptibility loci in ethnically diverse populations. Nature Genet. 15: 389-392, 1997. Note: Erratum: Nature Genet. 52: 1433 only, 2020.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: C5

Background:

Synonyms: B-lymphocyte antigen CD19, Differentiation antigen CD19, CD19, Cd19

Background: Complement component 5 is a protein that in humans is encoded by the C5 gene. This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mice with a homozygous mutation in this gene exhibit impaired bone fracture healing and an enhanced inflammatory response in an allergic lung disease model.

Molecular Weight: 75 kDa

Gene ID: 15139

UniProt: P06684

Pathways: Complement System, Carbohydrate Homeostasis