CD14 antibody (AA 20-259)

Catalog No. ABIN7600531

The Rabbit Polyclonal anti-CD14 antibody is suitable to detect CD14 in samples from Human. It has been validated for FACS, IHC, IF, ELISA and ICC.

Quick Overview for CD14 antibody (AA 20-259) (ABIN7600531)

Target: CD14

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-CD14 Antibody

Binding Specificity: AA 20-259

Purpose: Anti-CD14 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD14 Antibody (ABIN7600531). Tested in ELISA, Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD14 recombinant protein (Position: T20-N259).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background:

Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Cd14,

Tissue Specificity: Detected on peritoneal macrophages (at protein level) (PubMed:8612135). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level) (PubMed:19362712).

Background: CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Molecular Weight: 50 kDa

Gene ID: 929

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades