MPG antibody (AA 20-298)

Catalog No. ABIN7600539

This Rabbit Polyclonal antibody specifically detects MPG in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for MPG antibody (AA 20-298) (ABIN7600539)

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MPG Antibody

Binding Specificity: AA 20-298

Purpose: Anti-AAG/MPG Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AAG/MPG Antibody Picoband® (ABIN7600539). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AAG/MPG recombinant protein (Position: Q20-A298).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Boosalis, M., Derfler, B., Call, K., Samson, L. Cloning of a human 3-methyladenine DNA glycosylase cDNA whose gene maps to human chromosome 16. (Abstract) Am. J. Hum. Genet. 49 (suppl.): 399 only, 1991. 2. Engelward, B. P., Weeda, G., Wyatt, M. D., Broekhof, J. L. M., de Wit, J., Donker, I., Allan, J. M., Gold, B., Hoeijmakers, J. H. J., Samson, L. D.Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase. Proc. Nat. Acad. Sci. 94: 13087-13092, 1997. 3. Kielman, M. F., Smits, R., Bernini, L. F. Localization and characterization of the mouse alpha-globin locus control region. Genomics 21: 431-433, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MPG

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Alternative Name: MPG

Background:

Synonyms: Signal transducer and activator of transcription 1-alpha/beta,Transcription factor ISGF-3 components p91/p84,STAT1,

Tissue Specificity: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Background: MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.TheMPGgene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized humanMPGgene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,Hang et al.(1997)biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine(meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

Molecular Weight: 37 kDa

Gene ID: 4350

UniProt: P29372

Pathways: DNA Damage Repair