MPG antibody (AA 20-298)

Catalog No. ABIN7600539

This Rabbit Polyclonal antibody specifically detects MPG in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for MPG antibody (AA 20-298) (ABIN7600539)

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MPG Antibody

Binding Specificity: AA 20-298

Purpose: Anti-AAG/MPG Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AAG/MPG Antibody (ABIN7600539). Tested in ELISA, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AAG/MPG recombinant protein (Position: Q20-A298).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human
ELISA, 0.1-0.5 μg/mL/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MPG

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Alternative Name: MPG

Background:

Synonyms: Signal transducer and activator of transcription 1-alpha/beta,Transcription factor ISGF-3 components p91/p84,STAT1,

Tissue Specificity: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Background: MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.TheMPGgene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized humanMPGgene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,Hang et al.(1997)biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine(meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

Molecular Weight: 37 kDa

Gene ID: 4350

UniProt: P29372

Pathways: DNA Damage Repair