SCO1 antibody (AA 21-301)

Catalog No. ABIN7600609

Product Details anti-SCO1 Antibody

Target: SCO1 (SCO1 Cytochrome C Oxidase Assembly Protein (SCO1))

Binding Specificity: AA 21-301

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Anti-SCO1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SCO1 Antibody Picoband® (ABIN7600609). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SCO1 recombinant protein (Position: R21-S301).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Horvath, R., Lochmuller, H., Stucka, R., Yao, J., Shoubridge, E. A., Kim, S.-H., Gerbitz, K.-D., Jaksch, M. Characterization of human SCO1 and COX17 genes in mitochondrial cytochrome-c-oxidase deficiency. Biochem. Biophys. Res. Commun. 276: 530-533, 2000. 2. Leary, S. C., Cobine, P. A., Nishimura, T., Verdijk, R. M., de Krijger, R., de Coo, R., Tarnopolsky, M. A., Winge, D. R., Shoubridge, E. A. Cox19 mediates the transduction of a mitochondrial redox signal from SCO1 that regulates ATP7A-mediated cellular copper efflux. Molec. Biol. Cell 24: 683-691, 2013. 3. Leary, S. C., Kaufman, B. A., Pellecchia, G., Guercin, G.-H., Mattman, A., Jaksch, M., Shoubridge, E. A. Human SCO1 and SCO2 have independent, cooperative functions in copper delivery to cytochrome c oxidase. Hum. Molec. Genet. 13: 1839-1848, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SCO1

Target: SCO1 (SCO1 Cytochrome C Oxidase Assembly Protein (SCO1))

Alternative Name: SCO1 (SCO1 Products)

Background:

Synonyms: Peroxisome proliferator-activated receptor gamma coactivator 1-beta, PGC-1-beta, PPAR-gamma coactivator 1-beta, PPARGC-1-beta, PGC-1-related estrogen receptor alpha coactivator, PPARGC1B, PERC, PGC1, PGC1B, PPARGC1

Tissue Specificity: Preferentially expressed in melanocytes.

Background: Protein SCO1 homolog, mitochondrial, also known as SCO1, cytochrome c oxidase assembly protein, is a protein that in humans is encoded by the SCO1 gene. Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of cytochrome c oxidase), enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog to the yeast SCO1 gene.

Molecular Weight: 30 kDa

Gene ID: 6341

UniProt: O75880

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity