NCF1 antibody (AA 22-384)

Catalog No. ABIN7600691

This anti-NCF1 antibody is a Rabbit Polyclonal antibody detecting NCF1 in WB, IHC, ELISA, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for NCF1 antibody (AA 22-384) (ABIN7600691)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-NCF1 Antibody

Binding Specificity: AA 22-384

Purpose: Anti-p47 phox/NCF1 Antibody

Characteristics: Anti-p47 phox/NCF1 Antibody (ABIN7600691). Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human p47 phox/NCF1 recombinant protein (Position: Q22-R384). Human NCF1 shares 81% amino acid (aa) sequence identity with both mouse and rat NCF1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: NCF1

Background: Neutrophil cytosol factor 1, also known as p47phox, is a protein that in humans is encoded by the NCF1 gene. The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.

Molecular Weight: 40-50 kDa

Gene ID: 653361

UniProt: P14598

Pathways: PI3K-Akt Signaling