DEPTOR antibody (AA 22-409)

Catalog No. ABIN7600694

This anti-DEPTOR antibody is a Rabbit Polyclonal antibody detecting DEPTOR in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for DEPTOR antibody (AA 22-409) (ABIN7600694)

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEPTOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-DEPTOR Antibody

Binding Specificity: AA 22-409

Purpose: Anti-DEPDC6/DEPTOR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DEPDC6-DEPTOR Antibody Picoband® (ABIN7600694). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DEPDC6/DEPTOR recombinant protein (Position: A22-C409). Human DEPDC6/DEPTOR shares 97.9% amino acid (aa) sequence identity with mouse DEPDC6/DEPTOR.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/20/2009. 2. Peterson, T. R., Laplante, M., Thoreen, C. C., Sancak, Y., Kang, S. A., Kuehl, W. M., Gray, N. S., Sabatini, D. M. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival. Cell 137: 873-886, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DEPTOR

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Alternative Name: DEPTOR

Background:

Synonyms: DEPTOR, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6

Background: DEP domain-containing mTOR-interacting protein (DEPTOR) also known as DEP domain-containing protein 6 (DEPDC6) is a protein that in humans is encoded by the DEPTOR gene. Enables phosphatidic acid binding activity and protein serine/threonine kinase inhibitor activity. Involved in several processes, including negative regulation of TOR signaling, negative regulation of cell size, and negative regulation of protein kinase activity. Is active in lysosomal membrane.

Molecular Weight: 48 kDa

Gene ID: 64798

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Cell Size, BCR Signaling, Warburg Effect