MAPK12 antibody (AA 229-367)

Catalog No. ABIN7600727

This anti-MAPK12 antibody is a Rabbit Polyclonal antibody detecting MAPK12 in WB, ELISA, IF, FACS and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for MAPK12 antibody (AA 229-367) (ABIN7600727)

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-MAPK12 Antibody

Binding Specificity: AA 229-367

Purpose: Anti-p38 gamma/MAPK12 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-p38 gamma/MAPK12 Antibody Picoband® (ABIN7600727). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAPK12 recombinant protein (Position: S229-L367).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Goedert, M., Hasegawa, J., Craxton, M., Leversha, M. A., Clegg, S. Assignment of the human stress-activated protein kinase-3 gene (SAPK3) to chromosome 22q13.3 by fluorescence in situ hybridization. Genomics 41: 501-502, 1997. 2. Lechner, C., Zahalka, M. A., Giot, J.-F., Moller, N. P. H., Ullrich, A. ERK6, a mitogen-activated protein kinase involved in C2C12 myoblast differentiation. Proc. Nat. Acad. Sci. 93: 4355-4359, 1996. 3. Li, Z., Jiang, Y., Ulevitch, R. J., Han, J. The primary structure of p38-gamma: a new member of p38 group of MAP kinases. Biochem. Biophys. Res. Commun. 228: 334-340, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAPK12

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Alternative Name: MAPK12

Background:

Synonyms: Fos-related antigen 1, FRA-1, FOSL1, FRA1

Tissue Specificity: Adipocytes.

Background: Mitogen-activated protein kinase 12 (MAP kinase 12), also known as extracellular signal-regulated kinase 6 (ERK6) or stress-activated protein kinase 3 (SAPK3), is an enzyme that in humans is encoded by the MAPK12 gene. Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes.

Molecular Weight: 42 kDa

Gene ID: 6300

UniProt: P53778

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins