E2F1 antibody (AA 230-277)

Catalog No. ABIN7600771

The Rabbit Polyclonal anti-E2F1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect E2F1 in samples from Human.

Quick Overview for E2F1 antibody (AA 230-277) (ABIN7600771)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-E2F1 Antibody

Binding Specificity: AA 230-277

Purpose: Anti-E2F1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-E2F1 Antibody Picoband® (ABIN7600771). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human E2F1 recombinant protein (Position: Q230-D277).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, D., Pacal, M., Wenzel, P., Knoepfler, P. S., Leone, G., Bremner, R. Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925-929, 2009. 2. Chong, J.-L., Wenzel, P. L., Saenz-Robles, M. T., Nair, V., Ferrey, A., Hagan, J. P., Gomez, Y. M., Sharma, N., Chen, H.-Z., Ouseph, M., Wang, S.-H., Trikha, P., and 10 others. E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells. Nature 462: 930-934, 2009. 3. De Angelis, R., Iezzi, S., Bruno, T., Corbi, N., Di Padova, M., Floridi, A., Fanciulli, M., Passananti, C. Functional interaction of the subunit 3 of RNA polymerase II (RPB3) with transcription factor-4 (ATF4). FEBS Lett. 547: 15-19, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background:

Synonyms: Angiotensin-converting enzyme, ACE, Dipeptidyl carboxypeptidase I, Kininase II, CD143, Angiotensin-converting enzyme, soluble form, Ace, Dcp1

Tissue Specificity: Testis-specific isoform is expressed in spermatocytes, adult testis.

Background: Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Molecular Weight: 60 kDa

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy