RP2 antibody (AA 244-348)

Catalog No. ABIN7600869

Product Details anti-RP2 Antibody

Target: RP2 (Retinitis Pigmentosa 2 (X-Linked Recessive) (RP2))

Binding Specificity: AA 244-348

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Anti-RP2 Antibody Picoband® (monoclonal, 3D7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RP2 Antibody Picoband® (monoclonal, 3D7) (ABIN7600869). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human RP2recombinant protein (Position: D244-M348).

Clone: 3D7

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Chapple, J. P., Hardcastle, A. J., Grayson, C., Spackman, L. A., Willison, K. R., Cheetham, M. E.Mutations in the N-terminus of the X-linked retinitis pigmentosa protein RP2 interfere with the normal targeting of the protein to the plasma membrane. Hum. Molec. Genet. 9: 1919-1926, 2000. 2. Evans, R. J., Schwarz, N., Nagel-Wolfrum, K., Wolfrum, U., Hardcastle, A. J., Cheetham, M. E.The retinitis pigmentosa protein RP2links pericentriolar vesicle transport between the Golgi and the primary cilium. Hum. Molec. Genet. 19: 1358-1367, 2010. 

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RP2

Target: RP2 (Retinitis Pigmentosa 2 (X-Linked Recessive) (RP2))

Alternative Name: RP2 (RP2 Products)

Background:

Synonyms: Protein XRP2, RP2

Tissue Specificity: Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level).

Background: Protein XRP2 is a protein that in humans is encoded by the RP2 gene. It is mapped to Xp11.3. The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death.The RP2 protein is also involved in regulating the function and extension of outer segment of cone photoreceptors in mice.

Molecular Weight: 40 kDa

Gene ID: 6102

UniProt: O75695

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process