Aquaporin 4 antibody (AA 247-323)

Catalog No. ABIN7600876

The Rabbit Polyclonal anti-Aquaporin 4 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect Aquaporin 4 in samples from Human, Mouse and Rat.

Quick Overview for Aquaporin 4 antibody (AA 247-323) (ABIN7600876)

Target: Aquaporin 4 (AQP4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-Aquaporin 4 Antibody

Binding Specificity: AA 247-323

Purpose: Anti-Aquaporin 4/AQP4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Aquaporin 4/AQP4 Antibody Picoband® (ABIN7600876). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Aquaporin 4/AQP4 recombinant protein (Position: L247-V323).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Amiry-Moghaddam, M., Otsuka, T., Hurn, P. D., Traystman, R. J., Haug, F.-M., Froehner, S. C., Adams, M. E., Neely, J. D., Agre, P., Ottersen, O. P., Bhardwaj, A. : An alpha-syntrophin-dependent pool of AQP4 in astroglial end-feet confers biional water flow between blood and brain. Proc. Nat. Acad. Sci. 100: 2106-2111, 2003. 2. Agre P, Nielsen S (1996). "The aquaporin family of water channels in kidney". Nephrologie 17 (7): 409-15. 3. Lennon, V. A., Kryzer, T. J., Pittock, S. J., Verkman, A. S., Hinson, S. R. : IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J. Exp. Med. 202: 473-477, 2005. 4. Lu, M., Lee, M. D., Smith, B. L., Jung, J. S., Agre, P., Verdijk, M. A. J., Merkx, G., Rijss, J. P. L., Deen, P. M. T. : The human AQP4 gene: definition of the locus encoding two water channel polypeptides in brain. Proc. Nat. Acad. Sci. 93: 10908-10912, 1996. 5. Nagelhus EA, Mathiisen TM, Ottersen OP (2004). "Aquaporin-4 in the central nervous system: cellular and subcellular distribution and coexpression with KIR4.1". Neuroscience 129 (4): 905-13.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: AQP4

Background:

Synonyms: Retinal-specific ATP-binding cassette transporter,ATP-binding cassette sub-family A member 4,RIM ABC transporter,RIM protein,RmP,Stargardt disease protein,ABCA4,ABCR,

Tissue Specificity: Retinal-specific. Seems to be exclusively found in the rims of rod photoreceptor cells.

Background: Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0, the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

Molecular Weight: 32 kDa

Gene ID: 361

UniProt: P55087

Pathways: Sensory Perception of Sound