UBE2M antibody (AA 25-154)

Catalog No. ABIN7600885

The Rabbit Polyclonal anti-UBE2M antibody has been validated for WB, ELISA, IF, IHC, ICC and FACS. It is suitable to detect UBE2M in samples from Human, Mouse and Rat.

Quick Overview for UBE2M antibody (AA 25-154) (ABIN7600885)

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2M antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-UBE2M Antibody

Binding Specificity: AA 25-154

Purpose: Anti-UBC12/UBE2M Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-UBC12/UBE2M Antibody Picoband® (ABIN7600885). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UBC12/UBE2M recombinant protein (Position: K25-Q154).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Huang, D. T., Hunt, H. W., Zhuang, M., Ohi, M. D., Holton, J. M., Schulman, B. A. Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature 445: 394-398, 2007. 2. Osaka, F., Kawasaki, H., Aida, N., Saeki, M., Chiba, T., Kawashima, S., Tanaka, K., Kato, S. A new NEDD8-ligating system for cullin-4A. Genes Dev. 12: 2263-2268, 1998. 3. Scott, D. C., Monda, J. K., Bennett, E. J., Harper, J. W., Schulman, B. A. N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex. Science 334: 674-678, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for UBE2M

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBE2M

Background:

Synonyms: BAG family molecular chaperone regulator 5,BAG-5,Bcl-2-associated athanogene 5,BAG5,KIAA0873,

Tissue Specificity: Expressed in all tissues examined with lower levels in brain and testis.

Background: NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Molecular Weight: 21 kDa

Gene ID: 9040

UniProt: P61081