Melanoma gp100 antibody (AA 27-626)

Catalog No. ABIN7601027

This anti-Melanoma gp100 antibody is a Rabbit Polyclonal antibody detecting Melanoma gp100 in WB and ELISA. Suitable for Rat and Mouse.

Quick Overview for Melanoma gp100 antibody (AA 27-626) (ABIN7601027)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Melanoma gp100 Antibody

Binding Specificity: AA 27-626

Purpose: Anti-Melanoma gp100/Pmel Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Melanoma gp100/Pmel Picoband® Antibody (ABIN7601027). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Melanoma gp100/Pmel recombinant protein (Position: S27-V626).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Kubota, R., Wang, Y., Minoshima, S., Kudoh, J., Mashima, Y., Oguchi, Y., Shimizu, N. Mapping of the human gene for a melanocyte protein panel Pmel 17 (D12S53E) to chromosome 12q13-q14. Genomics 26: 430-431, 1995. 2. Kwon, B. S., Chintamaneni, C., Kozak, C. A., Copeland, N. G., Gilbert, D. J., Jenkins, N., Barton, D., Francke, U., Kobayashi, Y., Kim, K. K. A melanocyte-specific gene, Pmel 17, maps near the silver coat color locus on mouse chromosome 10 and is in a syntenic region on human chromosome 12. Proc. Nat. Acad. Sci. 88: 9228-9232, 1991. 3. Kwon, B. S., Halaban, R., Ponnazhagan, S., Kim, K., Chintamaneni, C., Bennett, D., Pickard, R. T. Mouse silver mutation is caused by a single base insertion in the putative cytoplasmic domain of Pmel 17. Nucleic Acids Res. 23: 154-158, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Pmel

Background:

Synonyms: Melanocyte protein PMEL, Melanocyte protein Pmel 17, Premelanosome protein, Silver locus protein, M-alpha, M-beta, Pmel, D10H12S53E, Pmel17, Si, Silv

Tissue Specificity: Ubiquitous.

Background: Melanocyte protein PMEL also known as premelanosome protein (PMEL) or silver locus protein homolog (SILV) is a protein that in humans is encoded by the PMEL gene. It is mapped to 10 D3, 10 77.13 cM. This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.

Molecular Weight: 95 kDa

Gene ID: 20431

UniProt: Q60696