LAT antibody (AA 28-235)

Catalog No. ABIN7601064

This Rabbit Polyclonal antibody specifically detects LAT in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for LAT antibody (AA 28-235) (ABIN7601064)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-LAT Antibody

Binding Specificity: AA 28-235

Purpose: Anti-LAT Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-LAT Antibody Picoband® (ABIN7599810). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LAT recombinant protein (Position: H28-E235). Human LAT shares 61% and 59.3% amino acid (aa) sequence identity with mouse and rat LAT, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aguado, E., Richelme, S., Nunez-Cruz, S., Miazek, A., Mura, A.-M., Richelme, M., Guo, X.-J., Sainty, D., He, H.-T., Malissen, B., Malissen, M. Induction of T helper type 2 immunity by a point mutation in the LAT adaptor. Science 296: 2036-2040, 2002. 2. Bacchelli, C., Moretti, F. A., Carmo, M., Adams, S., Stanescu, H. C., Pearce, K., Madkaikar, M., Gilmour, K. C., Nicholas, A. K., Woods, C. G., Kleta, R., Beales, P. L., Qasim, W., Gaspar, H. B. Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency. J. Allergy Clin. Immun. 139: 634-642, 2017. 3. Hundt, M., Tabata, H., Jeon, M.-S., Hayashi, K., Tanaka, Y., Krishna, R., De Giorgio, L., Liu, Y.-C., Fukata, M., Altman, A. Impaired activation and localization of LAT in anergic T cells as a consequence of a selective palmitoylation defect. Immunity 24: 513-522, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: The Linker for activation of T cells, also known as linker of activated T cells or LAT, is a protein involved in the T-cell antigen receptor signal transduction pathway which in humans is encoded by the LAT gene. The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 37 kDa

Gene ID: 27040

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway