NGFR antibody (AA 29-240)

Catalog No. ABIN7601116

The Rabbit Polyclonal anti-NGFR antibody has been validated for WB, FACS, IF, ELISA and ICC. It is suitable to detect NGFR in samples from Human and Rat.

Quick Overview for NGFR antibody (AA 29-240) (ABIN7601116)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-NGFR Antibody

Binding Specificity: AA 29-240

Purpose: Anti-p75 NGF Receptor/NGFR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-p75 NGF Receptor/NGFR Antibody Picoband® (ABIN7601116). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human p75 NGF Receptor/NGFR recombinant protein (Position: K29-Q240).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Beattie, M. S., Harrington, A. W., Lee, R., Kim, J. Y., Boyce, S. L., Longo, F. M., Bresnahan, J. C., Hempstead, B. L., Yoon, S. O. ProNGF induces p75-mediated death of oligodendrocytes following spinal cord injury. Neuron 36: 375-386, 2002. 2. Bentley, K. L., Ferguson-Smith, A. C., Miki, T., Kidd, K. K., Ruddle, F. H. Physical linkage of Hox 2.1 and nerve growth factor receptor. (Abstract) Cytogenet. Cell Genet. 51: 961 only, 1989. 3. Bibel, M., Barde, Y.-A. Neurotrophins: key regulators of cell fate and cell shape in the vertebrate nervous system. Genes Dev. 14: 2919-2937, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor P75ntr, Low-affinity nerve growth factor receptor, NGF receptor, p75 ICD, CD271, NGFR, TNFRSF16

Tissue Specificity: Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia.

Background: The low-affinity nerve growth factor receptor (nerve growth factor receptor (TNFR superfamily, member 16), also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors that stimulate neuronal cells to survive and differentiate. LNGFR is a member of the tumor necrosis factor receptor (TNF receptor)superfamily indeed, LNGFR was the first member of this large family of receptors to be characterized. It is mapped to 17q21.33. Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Molecular Weight: 45 kDa, 75 kDa

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding