PYCARD antibody (AA 3-193)

Catalog No. ABIN7601174

This anti-PYCARD antibody is a Rabbit Polyclonal antibody detecting PYCARD in WB, ELISA, IHC and FACS. Suitable for Mouse and Rat.

Quick Overview for PYCARD antibody (AA 3-193) (ABIN7601174)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PYCARD Antibody

Binding Specificity: AA 3-193

Purpose: Anti-TMS1/ASC/Pycard Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TMS1/ASC/Pycard Antibody Picoband® (ABIN7601174). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse TMS1/ASC/PYCARD recombinant protein (Position: R3-S193).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Agostini, L., Martinon, F., Burns, K., McDermott, M. F., Hawkins, P. N., Tschopp, J. NALP3 forms an IL-1-beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 20: 319-325, 2004. 2. Cai, X., Chen, J., Xu, H., Liu, S., Jiang, Q.-X., Halfmann, R., Chen, Z. J. Prion-like polymerization underlies signal transduction in antiviral immune defense and inflammasome activation. Cell 156: 1207-1222, 2014. 3. Conway, K. E., McConnell, B. B., Bowring, C. E., Donald, C. D., Warren, S. T., Vertino, P. M. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers. Cancer Res. 60: 6236-6242, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: Pycard

Background:

Synonyms: Transcription factor ETV6, ETS translocation variant 6, ETS-related protein Tel1, Tel, ETV6, TEL, TEL1

Tissue Specificity: Ubiquitous.

Background: PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm, however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 22-25 kDa

Gene ID: 66824

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome