Connexin 43/GJA1 antibody (AA 3-362)

Catalog No. ABIN7601189

This anti-Connexin 43/GJA1 antibody is a Rabbit Polyclonal antibody detecting Connexin 43/GJA1 in WB, IHC, ELISA, IF and ICC. Suitable for Human, Rat and Mouse.

Quick Overview for Connexin 43/GJA1 antibody (AA 3-362) (ABIN7601189)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: AA 3-362

Purpose: Anti-Connexin 43/GJA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Connexin 43/GJA1 Antibody Picoband® (ABIN7601189). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Connexin 43/GJA1 recombinant protein (Position: D3-R362).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Britz-Cunningham, S. H., Shah, M. M., Zuppan, C. W., Fletcher, W. H. : Mutations of the connexin43 gap-junction gene in patients with heart malformations and defects of laterality. New Eng. J. Med. 332: 1323-1329, 1995. 2. Liu, X. Z., Xia, X. J., Adams, J., Chen, Z. Y., Welch, K. O., Tekin, M., Ouyang, X. M., Kristiansen, A., Pandya, A., Balkany, T., Arnos, K. S., Nance, W. E. : Mutations in GJA1 (connexin 43) are associated with non-syndromic autosomal recessive deafness. Hum. Molec. Genet. 10: 2945-2951, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1

Background:

Synonyms: Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein, GJA1, GJAL

Tissue Specificity: Expressed in the heart and fetal cochlea.

Background: Connexin 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Molecular Weight: 43 kDa

Gene ID: 2697

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization