SIM2 antibody (AA 337-609)

Catalog No. ABIN7601370

This Rabbit Polyclonal antibody specifically detects SIM2 in ELISA, WB and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for SIM2 antibody (AA 337-609) (ABIN7601370)

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIM2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-SIM2 Antibody

Binding Specificity: AA 337-609

Purpose: Anti-SIM2 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human SIM2 shares 85.8% amino acid (aa) sequence identity with mouse SIM2.

Characteristics: Anti-SIM2 Antibody. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIM2 recombinant protein (Position: Y337-R609). Human SIM2 shares 85.8% amino acid (aa) sequence identity with mouse SIM2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SIM2

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Alternative Name: SIM2

Background:

Background: Single-minded homolog 2 is a protein that in humans is encoded by the SIM2 gene. This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.

Gene Full Name: SIM bHLH transcription factor 2

Molecular Weight: 65 kDa

Gene ID: 6493

UniProt: Q14190