Nicastrin antibody (AA 34-669)

Catalog No. ABIN7601402

The Rabbit Polyclonal anti-Nicastrin antibody (ABIN7601402) specifically detects Nicastrin in WB, ELISA, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Nicastrin antibody (AA 34-669) (ABIN7601402)

Target: Nicastrin (NCSTN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicastrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Nicastrin Antibody

Binding Specificity: AA 34-669

Purpose: Anti-Nicastrin/NCSTN Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-heavy chain Nicastrin/NCSTN Antibody (ABIN7601402). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Nicastrin/NCSTN recombinant protein (Position: N34-E669).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Nicastrin

Target: Nicastrin (NCSTN)

Alternative Name: NCSTN

Background:

Synonyms: Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65

Tissue Specificity: Detected in thymus and in activated splenocytes.

Background: Nicastrin, also known asNCSTN, is aproteinthat is a part of the gamma secretaseprotein complex. It is mapped to 1q23.2. This gene is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the gamma secretaseprotein complex, all of which undergo significant post-translational modificationbefore becoming active in the cell. NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex.The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly.The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Nicastrin has also been identified as a regulator of neprilysin, anenzymeinvolved in the degradation of amyloid beta fragment.

Molecular Weight: 120 kDa

Gene ID: 23385

UniProt: Q92542

Pathways: Notch Signaling, Neurotrophin Signaling Pathway