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5HT1B Receptor antibody (AA 35-390)

The Rabbit Polyclonal anti-5HT1B Receptor antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect 5HT1B Receptor in samples from Human, Rat and Mouse.
Catalog No. ABIN7601425

Quick Overview for 5HT1B Receptor antibody (AA 35-390) (ABIN7601425)

Target

See all 5HT1B Receptor (HTR1B) Antibodies
5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Reactivity

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Human, Rat, Mouse

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This 5HT1B Receptor antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • Binding Specificity

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    AA 35-390

    Purpose

    Anti-5HT1B/HTR1B Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-5HT1B/HTR1B Antibody Picoband® (ABIN7601425). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human 5HT1B/HTR1B recombinant protein (Position: A35-S390).

    Isotype

    IgG
  • Application Notes

    Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Bartsch, T., Knight, Y. E., Goadsby, P. J. Activation of 5-HT(1B/1D) receptor in the periaqueductal gray inhibits nociception. Ann. Neurol. 56: 371-381, 2004. 2. Demchyshyn, L., Sunahara, R. K., Miller, K., Teitler, M., Hoffman, B. J., Kennedy, J. L., Seeman, P., Van Tol, H. H. M., Niznik, H. B. A human serotonin 1D receptor variant (5HT1D-beta) encoded by an intronless gene on chromosome 6. Proc. Nat. Acad. Sci. 89: 5522-5526, 1992. 3. Garcia-Nafria, J., Nehme, R., Edwards, P. C., Tate, C. G. Cryo-EM structure of the serotonin 5-HT(1B) receptor coupled to heterotrimeric G(o). Nature 558: 620-623, 2018.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

    Alternative Name

    HTR1B

    Background

    Synonyms: Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90, THY1

    Tissue Specificity: Detected adjacent to nodes of Ranvier in juxtaparanodal zones in spinal cord nerve fibers, but also in paranodal regions in some myelinated spinal cord axons (at protein level) (PubMed:11086297). Detected in the islet of Langerhans (PubMed:21483673).

    Background: 5-hydroxytryptamine receptor 1B also known as the 5-HT1B receptor is a protein that in humans is encoded by the HTR1B gene. The protein encoded by this intronless gene is a G-protein coupled receptor for serotonin (5-hydroxytryptamine). Ligand binding activates second messengers that inhibit the activity of adenylate cyclase and manage the release of serotonin, dopamine, and acetylcholine in the brain. The encoded protein may be involved in several neuropsychiatric disorders and therefore is often a target of antidepressant and other psychotherapeutic drugs.

    Molecular Weight

    50 kDa

    Gene ID

    3351

    UniProt

    P28222

    Pathways

    JAK-STAT Signaling, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteins
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