CRH antibody (AA 185-196)

Catalog No. ABIN726800

The Rabbit Polyclonal anti-CRH antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect CRH in samples from Human, Rat and Mouse. There are 2+ publications available.

Quick Overview for CRH antibody (AA 185-196) (ABIN726800)

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CRH Antibody

Binding Specificity: AA 185-196

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CRF

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CRH antibody (ABIN726800)

Balan, Warnock, Puche, Gondre-Lewis, June, Aurelian: "The GABAA Receptor α2 Subunit Activates a Neuronal TLR4 Signal in the Ventral Tegmental Area that Regulates Alcohol and Nicotine Abuse." in: Brain sciences, Vol. 8, Issue 4, (2018) (PubMed).

Balan, Warnock, Puche, Gondre-Lewis, Aurelian: "Innately activated TLR4 signal in the nucleus accumbens is sustained by CRF amplification loop and regulates impulsivity." in: Brain, behavior, and immunity, (2017) (PubMed).

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: CRF

Background:

Synonyms: Corticoliberin, Corticoliberin precursor, Corticotropin releasing factor, Corticotropin releasing hormone, Corticotropin releasing hormone deficiency included, crf, crh, crh deficiency included, corticoliberin preproprotein, CRF_HUMAN, Corticotropin-releasing factor, CRF, CRH, Corticotropin-releasing hormone.

Background: Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010].

Gene ID: 1392

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour