FRK antibody (AA 39-339)

Catalog No. ABIN7601577

This Rabbit Polyclonal antibody specifically detects FRK in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FRK antibody (AA 39-339) (ABIN7601577)

Target: FRK (Fyn-Related Kinase (FRK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FRK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-FRK Antibody

Binding Specificity: AA 39-339

Purpose: Anti-FRK Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FRK Antibody Picoband® (ABIN7601577). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FRK recombinant protein (Position: Q39-A339).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Anneren, C., Reedquist, K. A., Bos, J. L., Welsh, M. GTK, a Src-related tyrosine kinase, induces nerve growth factor-independent neurite outgrowth in PC12 cells through activation of the Rap1 pathway: relationship to Shb tyrosine phosphorylation and elevated levels of focal adhesion kinase. J. Biol. Chem. 275: 29153-29161, 2000. 2. Cance, W. G., Craven, R. J., Bergman, M., Xu, L., Alitalo, K., Liu, E. T. Rak, a novel nuclear tyrosine kinase expressed in epithelial cells. Cell Growth Differ. 5: 1347-1355, 1994. 3. Cance, W. G., Craven, R. J., Weiner, T. M., Liu, E. T. Novel protein kinases expressed in human breast cancer. Int. J. Cancer 54: 571-

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FRK

Target: FRK (Fyn-Related Kinase (FRK))

Alternative Name: FRK

Background:

Synonyms: Carbonic anhydrase 12, Carbonate dehydratase XII, Carbonic anhydrase XII, CA-XII, Tumor antigen HOM-RCC-3.1.3, CA12

Tissue Specificity: Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677).

Background: Fyn-related kinase (Frk) is a tyrosine kinase of the Src family. Also known as Rak, Gtk, and Iyk, Frk is predominantly expressed in epithelial tissues. Frk protein is 49 % and 47 % identical to Fyn and Csk, respectively. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

Molecular Weight: 58 kDa

Gene ID: 2444

UniProt: P42685