SPRED2 antibody (AA 40-298)

Catalog No. ABIN7601643

This Rabbit Polyclonal antibody specifically detects SPRED2 in WB, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPRED2 antibody (AA 40-298) (ABIN7601643)

Target: SPRED2 (Sprouty-Related, EVH1 Domain Containing 2 (SPRED2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRED2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SPRED2 Antibody

Binding Specificity: AA 40-298

Purpose: Anti-SPRED2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SPRED2 Antibody Picoband® (ABIN7601643). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPRED2 recombinant protein (Position: R40-R298).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bundschu, K., Knobeloch, K.-P., Ullrich, M., Schinke, T., Amling, M., Engelhardt, C. M., Renne, T., Walter, U., Schuh, K. Gene disruption of Spred-2 causes dwarfism. J. Biol. Chem. 280: 28572-28580, 2005. 2. Kato, R., Nonami, A., Taketomi, T., Wakioka, T., Kuroiwa, A., Matsuda, Y., Yoshimura, A. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun. 302: 767-772, 2003. 3. Motta, M., Fasano, G., Gredy, S., Brinkmann, J., Bonnard, A. A., Simsek-Kiper, P. O., Gulec, E. Y., Essaddam, L., Utine, G. E., Guarnetti Prandi, I., Venditti, M., Pantaleoni, F., and 21 others. SPRED2 loss-of-function causes a recessive Noonan syndrome-like phenotype. Am. J. Hum. Genet. 108: 2112-2129, 2021.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SPRED2

Target: SPRED2 (Sprouty-Related, EVH1 Domain Containing 2 (SPRED2))

Alternative Name: SPRED2

Background:

Synonyms: Rho-related GTP-binding protein Rho6,Rho family GTPase 1,Rnd1,RND1,RHO6,

Tissue Specificity: Mostly expressed in brain and liver.

Background: Sprouty-related, EVH1 domain-containing protein 2 is a protein that in humans is encoded by the SPRED2 gene. SPRED2 is a member of the Sprouty (see SPRY1, MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade.

Molecular Weight: 53 kDa

Gene ID: 200734

UniProt: Q7Z698