MCF2 antibody (AA 42-925)

Catalog No. ABIN7601702

This anti-MCF2 antibody is a Rabbit Polyclonal antibody detecting MCF2 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for MCF2 antibody (AA 42-925) (ABIN7601702)

Target: MCF2 (MCF.2 Cell Line Derived Transforming Sequence (MCF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-MCF2 Antibody

Binding Specificity: AA 42-925

Purpose: Anti-DBL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DBL Antibody Picoband® (ABIN7601702). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DBL recombinant protein (Position: D42-Y925).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Anson, D. S., Blake, D. J., Winship, P. R., Birnbaum, D., Brownlee, G. G. Nullisomic deletion of the mcf.2 transforming gene in two haemophilia B patients. EMBO J. 7: 2795-2799, 1988. 2. Eva, A., Aaronson, S. A. Isolation of a new human oncogene from a diffuse B-cell lymphoma. Nature 316: 273-275, 1985. 3. Eva, A., Vecchio, G., Rao, C. D., Tronick, S. R., Aaronson, S. A. The predicted DBL oncogene product defines a distinct class of transforming proteins. Proc. Nat. Acad. Sci. 85: 2061-2065, 1988.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MCF2

Target: MCF2 (MCF.2 Cell Line Derived Transforming Sequence (MCF2))

Alternative Name: MCF2

Background:

Synonyms: Protein phosphatase 1 regulatory subunit 14A,17 kDa PKC-potentiated inhibitory protein of PP1,Protein kinase C-potentiated inhibitor protein of 17 kDa,CPI-17,PPP1R14A,CPI17, PPP1INL,

Tissue Specificity: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .

Background: The DBL proto-oncogene is a protein that in humans is encoded by the MCF2 gene. The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.

Molecular Weight: 125 kDa

Gene ID: 4168

UniProt: P10911